Flow Cytometry, Cell Sorting, and Cell Analysis

This core provides investigators with multi-parameter flow cytometric analysis capabilities, training and service. Highly trained research staff are at hand, as are dedicated workstations with FloJo and Winlist software systems. In parallel, we also provide services in cell separation procedures including fluorescence activated cell sorting via an upgraded FACS ARIA II system, and magnetic activated cell sorting systems (including large-scale AutoMACS procedures).

Training and/or services in a number of cell-based assays also are provided. These include multiwell fluorimetry and the luminometry, Evos microscopy, Vicell analysis, cytospin preparations and processing, and colony forming assay scanning and quantitative analyses.

Services, Training and Resources Available:

Instruments

• MACS Quant Flow Cytometer
• FACS Aria Multi Parameter Cell Sorter
• Stem Vision
• autoMACS
• Turner Fluorimeter- MOD Microplate Reader
• K2 Cellometer
• Cytospin system
• Electroporation units

Flow cytometry training modules

• Basic concepts & instrument components
• Computer interfaces & basic analysis data (and storage)
• Lasers, fluorescent labels
• Up to 9 color analyses
• Cell cycle analysis and software
• MACS Quantify
• Flow Jo

Technician assisted services

• MACS Quant Flow Cytometer
• Fluorimeter- Modulers Microplate Reader
• K2 Cellometer
• Stem Vision
• Cytospin System
• autoMACS
• FACsAria

Cell Separation:

• Service contracted core instruments, and the instrument reagents such as sheath fluid and running buffers
• Technician performed FACSAria cell sorting upon request
• Individual use of the autoMACS
• FACSAria overview and autoMACS training for interested individuals
• Technical assistance in cell preparation for cell sorting