Equipment & Services Available
The Molecular Phenotyping core provides instrumentation, training, and services for in vitro cell phenotyping.
Agilent 2100 Bioanalyzer: With automated electrophoresis, the Agilent 2100 Bioanalyzer system provides sizing, quantitation, and purity assessments for DNA, RNA, and protein samples.[Learn More]
FLUIDIGM C1: Single cell isolation for mRNA sequencing, qPCR, DNA sequencing, epigenetics or miRNA expression. [Learn More]
NANODROP 2000: UV-Vis spectrophotometry
DIAGENODE BIORUPTOR 300: Chromatin shearing for chromatin
immunoprecipitation (ChIP), genomic DNA shearing for next generation
sequencing, RNA shearing, cell and tissue disruption, FFPE DNA extraction. [Learn More]
BIORAD CFX CONNECT, CFX 384: Real-time PCR (SYBR and TaqMan)
LEICA DM IRB microscope: 4 color inverted epifluorescence microscopy. [Learn More]
LEICA DMI6000B fitted with BL-X culture chamber: Fully automated 4 color epifluorescence live-cell microscopy
BIOMEK 3000: Automated liquid handling
QIAcube: Fully automated sample prep using QIAGEN spin-column kits (DNA, RNA, or protein)
| Service | Cost |
|---|---|
| Agilent 2100 Bioanalyzer | Contact Michele Karolak for pricing details |
| Fluidigm C1: C1 Cell Prep | Contact Michele Karolak for pricing details |
| Fluidigm C1: Preamp for DeltaGene | Contact Michele Karolak for pricing details |
| Fluidigm C1: Preamp for TaqMan | Contact Michele Karolak for pricing details |
| Fluidigm C1: Library for mRNA seq | Contact Michele Karolak for pricing details |
| BIORAD CFX Connect, CFX 384, MyIQ, and iQ5 | $3.50/hour |
| Biomek 3000 (not including consumables) | $10/run |
| Nanodrop 2000 | $2/run |
| Leica DM IRB (Inverted epifluorescence microscopy) | $5/hour |
| Leica DMI 6000B (Epifluorescence live cell microscopy) | $10/hour |
| QIAcube: RNeasy Micro | $10.82/sample |
| QIAcube: RNeay Mini | $6.96/sample |
| QIAcube: DNeasy Blood and Tissue | $4.05/sample |
| QIAcube:QIAprep Spin Miniprep | $2.69/sample |
| Diagenode Bioruptor | $5/run |
| Tissue Homogenizer | $20/50g Zirconium beads |
Contact Us
Director: Aaron Brown, PhD
Aaron.C.Brown@mainehealth.org
Manager: Michele Karolak
Michele.Karolak@mainehealth.org
Request Training
Users must be appropriately trained before using core equipment.
Instrument training: contact Michele Karolak.
Workstation training: contact Dr. Brown
